FastQCFastQC Report
Mon 27 Feb 2023
SRR3127225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13709240
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5307203.8712576335376725No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT611350.44594011046564214No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT261680.19087856073713788No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT259330.18916438839789806No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT257420.18777116747536698No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT248000.18089988941764826No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG228780.1668801479877805TruSeq Adapter, Index 22 (95% over 21bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT226120.16493985078676862No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158790.11582698967995309No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC143270.10450615789059058No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA142720.10410496861970467No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC137310.10015872506426322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1545650.090.157231
ATAGGGC59550.070.899273
AGGGACT333350.070.5066155
GACTTAA274200.069.668678
ACTTAAT277950.068.4582069
ATAGGGA83750.068.320813
GGACTTA284600.067.948427
AGTAGGG189750.067.52512
GGTAAGG72950.066.755291
GGATAGG70200.066.555311
ATAGAGG86400.066.438021
AAGGGGC465100.065.798854
GTTTTTT2135450.065.6178362
AATAGGG167150.065.599822
GTAGGGA91450.065.190263
GTATAGG40450.064.7898941
TATAGGG94500.064.782792
TAGGGAC59900.064.598644
TAGTAGG45250.064.4679261
ATAAGGG161050.064.289062
Produced by FastQC (version 0.11.5)