FastQCFastQC Report
Mon 27 Feb 2023
SRR3127224.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127224.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3004968
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321341.0693624690845294No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57050.18985227130538493No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC53280.17730638063367066No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG41370.13767201514292332No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG35020.1165403425261101No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC34220.11387808455863757Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100750.084.276671
ATAGGGC18250.073.1359943
AGTAGGG50050.070.238182
ATAGAGG20150.069.763921
AGGGATG52850.069.7252665
AGGGAAT36600.069.604495
TAGGGCA15300.069.122184
CGTAGGG7650.068.8069462
TAGAGGG53350.068.7125242
AAGGGAC31050.068.423164
ACGGGTA3050.067.807724
TGTAGGG23400.067.483742
AGGGTAC10500.067.146135
AGATAGG17450.067.087041
ATAGGGA26650.067.0134663
AATAGGG46650.066.6932752
GAATAGG18800.066.5209661
ACGGGAT7300.066.319414
AGGGCAT27150.066.305265
GGTAAGG17800.066.2961651
Produced by FastQC (version 0.11.5)