FastQCFastQC Report
Mon 27 Feb 2023
SRR3127223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399025
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234380.9769802315524014No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45110.18803472243932431No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG39450.16444180448307125No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39440.16440012088244182No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG30260.12613457550463208No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC30090.1254259542939319Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74800.084.96111
ATAGGGC14150.074.259723
ACGGGTA2550.073.913654
GAATAGG12950.072.446111
AGGGAAT29600.072.237785
CGTAGGG5500.071.966522
ATAAGGG28700.071.4202352
AGGGATG41600.071.282145
AGGGTAC8500.070.767815
TAAGGGA21700.070.354213
GGTAAGG14050.070.129671
TAGGGCA11700.070.075834
ATAGCGG4200.069.594391
ATAGAGG15650.069.587211
TAGAGGG42900.069.308422
TATAGGG16850.068.2344742
CTACGAC10200.068.187745
AGTAGGG40950.067.890972
GTACGGG5150.067.707752
ACGGGAT6900.067.606894
Produced by FastQC (version 0.11.5)