FastQCFastQC Report
Mon 27 Feb 2023
SRR3127222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497842
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246550.9870520233065182No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47960.19200573935421056No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43000.17214859867037227No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG38720.1550138079189957No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC29960.11994353525963612Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG29570.11838218750425368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74650.085.1189041
AGGGATG40400.071.7750245
ATAGGGA21250.071.214193
AGTAGGG43400.070.959812
GACCGAT2400.070.49548
TAAGGGA22750.070.03063
AGGGAAT30150.069.832935
ATAAGGG35450.069.498572
GGTAAGG14800.069.329311
ACGGGAT6700.068.741794
ATAGGGC14650.068.650823
ACGGGTA2400.068.537194
GTAGGGA21950.068.51493
GAATAGG16300.068.4357151
CGTAGGG7050.068.025532
AGAGGGC35450.067.346813
AAGGGAC25350.067.2973864
GTAAGGG40450.067.0684362
GTACGGG6250.066.9531252
AGACGGG16650.066.643632
Produced by FastQC (version 0.11.5)