FastQCFastQC Report
Mon 27 Feb 2023
SRR3127221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3973650
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT544101.3692700665634872No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC145960.36731971864658436No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC55740.1402740553395493Illumina Single End Adapter 1 (95% over 21bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC50620.12738917619820567No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG50490.1270620210637575TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG47280.11898380582084482TruSeq Adapter, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT176950.086.351911
AGGGATG105950.080.206755
ATAGGGA46400.075.0571063
CGTAGGG9900.075.0071262
CTACGAC29150.074.493245
GTAGGGC18800.073.748943
ACGGGAT16100.073.276844
AAGGGAT92450.073.2615364
TAAGGGA43400.072.773153
GGATGGC80450.072.619477
GAGGGAT71650.072.291014
ATAGCGG8650.072.289411
TAGGGCA24200.072.057214
GTAGGGA35950.071.773653
CGGGATG20400.071.6545265
TCCGCTA30600.071.291161
AGAGGGC55450.071.198253
AGGGAAT62800.070.652225
GAGGGTA24150.070.649394
ACGACCA29900.070.5802467
Produced by FastQC (version 0.11.5)