FastQCFastQC Report
Mon 27 Feb 2023
SRR3127220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175652
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402711.2681175393273572No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC109160.3437404350350731No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC51420.16191950503392688Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG46590.14671002994030832TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG45220.142395955224313TruSeq Adapter, Index 23 (95% over 23bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC39760.1252026355532659No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT135350.086.831141
AGGGATG83900.080.992345
TAGGGCA20350.075.506624
AGGGAAT49000.075.0936665
GTAGGGA30700.074.769163
AGGGTAC15150.074.75535
GGATGGC64600.074.272987
CGTAGGG8600.073.989012
AAGGGAT67500.073.668294
CTACGAC22950.073.51055
CGAGGGA20650.072.811933
CGGGATG16550.072.690825
TAAGGGA32950.072.6674963
ACGGGAT12150.072.543284
GAGGGAT55500.072.526514
ATAGGGA37050.072.259023
CGCTACG23550.072.051583
AAGGGCG13050.071.874444
GGTAAGG18050.071.852391
GAGGGTA18950.071.135884
Produced by FastQC (version 0.11.5)