FastQCFastQC Report
Mon 27 Feb 2023
SRR3127219.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127219.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352480
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418491.248299766143273No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC116620.3478618813535055No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC51020.1521858445091395Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG45570.1359292225456975TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG45530.1358099078890851TruSeq Adapter, Index 23 (95% over 23bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC41880.12492244547320193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT142950.086.653781
AGGGATG83300.079.3262255
CGTAGGG8900.075.5480652
AGGGAAT53150.074.365115
ATGCGGG13700.074.132822
AAGGGAT70350.073.486084
ATAGGGA41800.073.3073963
GGATGGC64950.073.228117
GTACGGG8450.072.894022
ACGGGAT12450.072.855864
ACGGGTA4400.072.632794
ATAGCGG6700.072.351611
GAGGGAT55750.072.330084
AGGGCAT33800.072.304145
GTAGGGA32300.072.169833
TAGGGCA20950.072.010784
CGGGATG16700.071.762945
GAGGGTA18600.071.759914
AAGGGCG15300.070.957294
AAGGGCA53250.070.695114
Produced by FastQC (version 0.11.5)