FastQCFastQC Report
Mon 27 Feb 2023
SRR3127218.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127218.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277554
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360961.1013090859830228No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG62240.18989771030469674No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC58540.17860880400444964No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG49840.1520646189200849No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45350.13836537857194725No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39720.12118793466103077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103450.083.938671
ACGGGAT8650.072.8151254
CGTAGGG10050.072.0169142
TAAGGGA28650.070.8672563
TAGGGCA16900.070.088494
GTAGGGT16050.069.692883
GAATAGG19300.069.4245761
ATAGGGA29100.069.286853
AGGGATG46250.068.903975
TATAGGG26200.067.985732
ATAGGGC20050.067.978273
AGGGTAC13900.067.630325
AGAGGGC46700.067.529293
AAGGGTA22800.067.001024
GTAGGGC13400.066.990783
TAGAGGG54550.066.943152
AGGGCAT30350.066.129535
TAGGGCG4550.066.115224
AGTAGGG46400.066.040372
GGTAAGG17250.065.955641
Produced by FastQC (version 0.11.5)