FastQCFastQC Report
Mon 27 Feb 2023
SRR3127216.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127216.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748244
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274370.9983465805801814No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG54930.19987308259383085No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC45900.16701573804945993No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG42340.15406201196109223No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36980.13455864908647122No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32580.11854842583118529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78000.083.085191
TAAGGGA22750.073.33423
TAGGGCG4000.072.843584
TAGGGCA14750.071.369984
GAATAGG15200.070.595261
ATAGGGA25600.070.310223
AGGGATG40700.069.858725
ATAGGGC17500.069.822423
ACGGGTA2050.068.774434
AGGGAAT33850.067.8906255
TAGGGTA10200.067.7293244
GACCGAT2500.067.674048
CGTAGGG7600.067.4314042
ACGGGAT7050.067.327414
ATAAGGG36300.066.833212
AGTAAGG16500.066.7446061
AGTAGGG41050.066.659052
TATAGGG20600.066.644592
TACGAGG5400.066.2375261
GTAGGGC12000.066.185843
Produced by FastQC (version 0.11.5)