FastQCFastQC Report
Mon 27 Feb 2023
SRR3127215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949013
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144351.5210539792394835No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG38540.40610613342493723TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG36380.38334564436946594TruSeq Adapter, Index 27 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33850.35668636783690005No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC31430.33118619028401086TruSeq Adapter, Index 27 (100% over 24bp)
AAAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT13440.14162082078959928No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT12100.1275008877644458TruSeq Adapter, Index 27 (100% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12020.12665790668831722No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT11890.1252880624396083TruSeq Adapter, Index 27 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41200.086.037281
TAGGGTA3200.082.254374
AGGGTAC3100.080.354885
TCCGCTA7850.079.052721
AGGGCAT9200.078.673965
CGCTACG7850.078.433173
CGGGTAT1150.077.6522145
AGGGATG19500.077.61035
GGGTACG551.8189894E-1276.9091346
GCTACGA7950.076.268224
AAGGGTA6350.076.240284
GGATGGC12750.075.93737
CTACGAC8100.074.271655
AAGGGAC9750.074.239854
CGTAGGG1650.074.0606462
AAGGGCA13900.074.0543064
TAGGGCA5150.073.0135964
AGTAAGG5550.072.848071
AGGGTAT6800.072.573575
ACGACCA7850.072.44597
Produced by FastQC (version 0.11.5)