FastQCFastQC Report
Mon 27 Feb 2023
SRR3127214.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127214.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783485
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104821.3378686254363517No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG38690.49381928179863044TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG31330.39988002322954486TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC28200.35993031136524634TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26100.33312699030613224No Hit
AAAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT12450.15890540342189063No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT11870.1515025814150877TruSeq Adapter, Index 27 (100% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT10980.1401430786805108TruSeq Adapter, Index 27 (100% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT10890.13899436492083447TruSeq Adapter, Index 27 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9480.12099784935257216No Hit
AGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT8900.11359502734576922TruSeq Adapter, Index 27 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG8320.10619220533896628TruSeq Adapter, Index 27 (100% over 23bp)
GGAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT8170.10427768240617243TruSeq Adapter, Index 27 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT7890.1007039062649572TruSeq Adapter, Index 27 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT28950.088.2359851
TAGGGTA2600.085.163364
CGTAGGG1700.080.366922
AGGGATG16450.078.847725
TAGGGCG1800.078.519414
AGGGTAC2700.078.3239755
CGGGAAT2100.078.3239755
AGGGCAT7350.077.364915
TAAGGGA5150.076.842293
GTAGCGG1350.076.8040161
AAGGGTA5200.076.1034244
CTAAGGG2550.075.748132
AGTAGGG10950.075.7228242
AGAGGGC10100.075.565213
AAGGGAC7150.075.1150744
TAGGGTC2400.074.593444
AAGGGCA10950.074.432094
AGGGAAT15000.074.251135
TAGCGGG2800.074.0325852
CGCTACG5750.073.739963
Produced by FastQC (version 0.11.5)