FastQCFastQC Report
Mon 27 Feb 2023
SRR3127213.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127213.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805786
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107601.335342137987009No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG36150.44863028148912987TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG30110.3736724142638368TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC28270.35083756729454224TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27310.33892373409317117No Hit
AAAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT11920.14793009558369097No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT11510.14284189598727204TruSeq Adapter, Index 27 (100% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT11290.14011164254529118TruSeq Adapter, Index 27 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT10480.13005934578163433TruSeq Adapter, Index 27 (100% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9780.1213721757389679No Hit
AGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT8620.10697629395397786TruSeq Adapter, Index 27 (95% over 21bp)
GGAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT8110.10064707006574947TruSeq Adapter, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAC2600.090.37965
CGTTTTT28300.088.1256941
CGTAGGG1550.087.9633562
GGGTACG353.864625E-880.566966
TAGGGTA2000.079.895574
AAGGGTA5500.079.468324
AAGGGCG1850.078.752394
AGGGCAT7650.078.021825
TAAGGGA5400.077.4586643
CGAAGGG6450.077.264792
AGGGAAT15000.077.075725
GTAGGGC2750.076.904833
AGGGATG15650.075.9766165
AAGGGCA12550.075.270724
CTAAGGG3200.074.932
TAGGGCG1200.074.4125444
GTAGGGA5450.074.1610263
ATAAGGG10350.074.0427862
GATAAGG4650.073.8725361
GGATGGC9750.073.7497567
Produced by FastQC (version 0.11.5)