FastQCFastQC Report
Mon 27 Feb 2023
SRR3127212.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127212.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736562
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101301.3753085279990007No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG32740.4444975439949386TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32190.4370304197066913No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG30780.4178874283495483TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC30200.41001300637285115TruSeq Adapter, Index 20 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT11260.1528723990648445No Hit
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT10500.14255419095744826TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT10310.1399746389305992TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG8890.12069588167730619TruSeq Adapter, Index 20 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT7990.10847695102381064No Hit
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT7630.10358937876241239TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG353.583409E-1094.025061
ATAGCGG1150.085.848971
CGTTTTT34450.085.700641
GGTAAGG3400.084.346011
CGGGTAT1150.081.738715
CGCTACG7050.080.660783
TAGGGCA3150.080.571024
TCCGCTA7300.080.500911
AGGGATG16900.080.3723755
GGAGCTA37800.080.1980069
GTAGGGT1600.079.306723
CTACGAC7250.079.089255
GGGCGAT2500.078.95966
AGGGAAT16150.078.866485
TTAATCG301.4556626E-678.332949
GGATGGC13400.078.216037
GCGGGAT2350.077.999614
ACGGGAT2050.077.950824
TAGGGTA2000.077.549614
GTAGGGC2850.077.503123
Produced by FastQC (version 0.11.5)