FastQCFastQC Report
Mon 27 Feb 2023
SRR3127211.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127211.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602591
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71721.1901936802906117No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG31850.528550874473731TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC27590.4578561578251252TruSeq Adapter, Index 20 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG27240.45204790645728193TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24620.4085689962179986No Hit
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT10350.1717582904490774TruSeq Adapter, Index 20 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT9690.16080558786971594No Hit
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT9340.15499733650187275TruSeq Adapter, Index 20 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT8750.14520628419607992No Hit
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG7910.13126648091325624TruSeq Adapter, Index 20 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT7020.11649692743502642TruSeq Adapter, Index 20 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT7010.11633097739594518No Hit
GGAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT6280.10421662454301509No Hit
GATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC6160.10222522407404026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA800.088.3064963
CGTTTTT24700.086.778911
ACGGGTA550.085.630544
GCTACGA4900.085.543174
CTACGAC4950.084.49645
AGGGATG11600.083.051845
CGGGTAT401.035005E-982.241585
ATAGCGG1150.081.927921
TAAACGG750.081.654821
TACGACC5150.081.214986
AGGGAAT14900.081.0588155
CGCTACG5200.080.607983
AGGGCAT5100.080.168275
TCCGCTA5250.079.860211
ACGACCA5150.079.389937
TAGGGCA2850.079.320934
CGACCAA5250.077.877748
GGATGGC9850.077.768697
AAGGGCA9750.077.287054
GGAGCTA23900.077.27669
Produced by FastQC (version 0.11.5)