FastQCFastQC Report
Mon 27 Feb 2023
SRR3127210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618200
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75281.2177288903267551No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG30690.49644128113879TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC25920.41928178582982856TruSeq Adapter, Index 20 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG25920.41928178582982856TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25730.416208346813329No Hit
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT10700.17308314461339372TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT9740.15755418958265935TruSeq Adapter, Index 20 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT9560.15464251051439665No Hit
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT8620.1394370753801359No Hit
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG7780.12584923972824327TruSeq Adapter, Index 20 (95% over 22bp)
AGAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT6950.1124231640245875No Hit
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT6550.10595276609511484TruSeq Adapter, Index 20 (95% over 21bp)
GGAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT6310.10207052733743126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG900.088.857921
CGTTTTT25200.087.924541
TACGGGT550.085.441753
TCCGCTA4750.085.171551
GGATAGG2400.084.284341
CGGGTAT750.081.4544755
AGGGAAT15000.080.8278965
GTAGGGT1050.080.5593643
CGCTACG4900.080.5593643
GGAATGC7600.080.38277
GCGTAGG1000.079.972131
CGAGGGA3000.079.888053
GGAGCTA30300.079.8725369
CTACGAC4950.079.745635
CGTAGGG1300.079.558722
ACGGGAT2600.079.526554
GCTACGA5000.078.948184
AGTACGG600.078.404041
CGGGATA1200.078.32165
GGATGGC10350.078.094597
Produced by FastQC (version 0.11.5)