FastQCFastQC Report
Mon 27 Feb 2023
SRR3127209.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127209.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585725
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68651.1720517307610228No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG26900.459259891587349No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG25780.44013829015322886No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC24330.41538264543941267No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT8730.1490460540355969No Hit
AGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT7990.13641213880233896No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT6870.11729053736821889No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG6240.10653463656152631No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT6120.10448589355072774No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5950.10158350761876307No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGC156.893111E-494.0253694
ACGGGTA950.093.985224
CGTAGGG1100.089.713172
CGTTTTT28850.086.373831
GTGTAGG1200.082.279231
TCGTAGG401.031367E-982.279221
CGCTACG1200.082.237083
AGGGCGT750.081.4538655
AGGGTAT4700.080.9872745
AGCGGGA2750.080.314653
CTACGAC1250.078.94765
AGTTGCG301.4566067E-678.321039
ACGGGAT1750.077.8734744
GTAGGGT1450.077.780883
AGGGTTA1650.076.8975
AGGACGG2100.076.122271
TCCGCTA1300.075.950051
GGAGCTA22050.075.8701559
TAGACGG507.094059E-1175.2267151
TAGTAGG2000.075.2267151
Produced by FastQC (version 0.11.5)