FastQCFastQC Report
Mon 27 Feb 2023
SRR3127204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734658
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295301.0798425250982024No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT55950.2045959677590397No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG41210.15069526061394148TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG36850.13475176786274554TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC35820.13098530053849514TruSeq Adapter, Index 21 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35220.12879124190300945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89100.084.886531
ATAGCGG5150.081.336111
ATAGGGC15700.072.141023
TAGGGAT18400.072.0271454
GAATAGG15600.071.804721
AGTAGGG46750.071.111692
TAAGGGA24100.070.397153
AGGGATG37150.070.210025
AGGGAAT30300.070.1068955
ATAGGGA22950.070.03393
GAGGGAT33700.069.448744
TAGCGGG14600.069.2450562
ACGGGAT7650.068.805244
ATGAGGG43800.068.064132
TAGGGCA13050.068.0638054
ATGCGGG13250.067.782982
AGAGGGC39000.067.723073
AAGAGGG92750.067.225312
CTACGAC8050.067.137775
TAGTAGG14150.066.190741
Produced by FastQC (version 0.11.5)