FastQCFastQC Report
Mon 27 Feb 2023
SRR3127203.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127203.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319778
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354071.0665472209286284No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC69510.20938147068870266No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG56300.1695896532840449TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG52760.1589262896494886TruSeq Adapter, Index 14 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT52270.1574502873384907No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45390.13672600999223442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT114900.084.181131
CGTAGGG9050.075.30272
AGTAGGG57300.071.52422
TAGGGCA16550.071.279864
AGGGATG52800.070.228695
AGGGCAT30750.069.846165
TAGAGGG53850.069.82252
CGAAGGG32250.069.806662
ATAGGGA31100.069.6677253
AAGGGTA25500.069.300884
AAGGGCG14850.068.046084
GGTAAGG20750.067.7569051
TAAGGGA29550.067.4371953
AGAGGGC49850.067.4111943
AGGGTAG18200.066.8805545
ATAGGGC21800.066.8338243
AAGAGGG110550.066.832072
GACCGAT2550.066.348918
GTAGGGC17500.066.336113
ATAGAGG22600.066.1636051
Produced by FastQC (version 0.11.5)