Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127202.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2550425 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 25619 | 1.0044992501249792 | No Hit |
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC | 6658 | 0.2610545301273317 | No Hit |
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG | 5187 | 0.20337786839448327 | TruSeq Adapter, Index 14 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG | 4818 | 0.18890969152200124 | TruSeq Adapter, Index 14 (95% over 21bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 3953 | 0.15499377554721272 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 3136 | 0.12295989884038934 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT | 2950 | 0.1156669966770244 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 8575 | 0.0 | 85.60915 | 1 |
TAGGGCA | 1235 | 0.0 | 75.17712 | 4 |
ACGGGAT | 660 | 0.0 | 73.54949 | 4 |
GTACGGG | 605 | 0.0 | 73.22923 | 2 |
ATAGGGA | 2095 | 0.0 | 71.5368 | 3 |
AGGGATG | 3780 | 0.0 | 70.863815 | 5 |
TAGAGGG | 3915 | 0.0 | 70.54688 | 2 |
AGAGGGC | 3630 | 0.0 | 70.23879 | 3 |
AGGGCAT | 2460 | 0.0 | 70.10879 | 5 |
CGTAGGG | 580 | 0.0 | 69.88475 | 2 |
AGTAAGG | 1570 | 0.0 | 69.671326 | 1 |
GAGGGTA | 1625 | 0.0 | 69.02556 | 4 |
GTAGGGA | 1940 | 0.0 | 68.99267 | 3 |
CGAAGGG | 2465 | 0.0 | 68.64194 | 2 |
AGGGAAT | 3400 | 0.0 | 68.55581 | 5 |
ATGCGGG | 1150 | 0.0 | 68.44325 | 2 |
AGGGACT | 3545 | 0.0 | 67.87272 | 5 |
ATAGAGG | 1670 | 0.0 | 67.75799 | 1 |
TAAGGGA | 1955 | 0.0 | 67.74011 | 3 |
AGTAGGG | 4120 | 0.0 | 67.723045 | 2 |