FastQCFastQC Report
Mon 27 Feb 2023
SRR3127202.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127202.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550425
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256191.0044992501249792No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC66580.2610545301273317No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG51870.20337786839448327TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG48180.18890969152200124TruSeq Adapter, Index 14 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39530.15499377554721272No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31360.12295989884038934No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT29500.1156669966770244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85750.085.609151
TAGGGCA12350.075.177124
ACGGGAT6600.073.549494
GTACGGG6050.073.229232
ATAGGGA20950.071.53683
AGGGATG37800.070.8638155
TAGAGGG39150.070.546882
AGAGGGC36300.070.238793
AGGGCAT24600.070.108795
CGTAGGG5800.069.884752
AGTAAGG15700.069.6713261
GAGGGTA16250.069.025564
GTAGGGA19400.068.992673
CGAAGGG24650.068.641942
AGGGAAT34000.068.555815
ATGCGGG11500.068.443252
AGGGACT35450.067.872725
ATAGAGG16700.067.757991
TAAGGGA19550.067.740113
AGTAGGG41200.067.7230452