FastQCFastQC Report
Mon 27 Feb 2023
SRR3127201.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127201.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619779
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265871.014856596682392No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC67510.25769349246634926No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG49160.18764941622938422TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG47610.18173288662898665TruSeq Adapter, Index 14 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41000.15650175072019434No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32920.12565945448070237No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT28330.10813889263178306No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81550.085.935811
CGTAGGG6800.073.975652
AGGGATG37700.073.174795
TAGGGCA11900.073.0616464
TAGGGCG3800.072.968124
ATAGGGA23350.072.255733
AGTAGGG40150.070.3724752
GGTAAGG14650.070.0361561
AGAGGGC41350.070.011583
ATAGCGG5400.069.726781
TAAGGGA21950.069.584663
AGGGCAT26550.069.5653155
GTAGGGA19150.069.451623
TAGCGGG11650.069.40912
CGAAGGG26850.068.986782
ATAGAGG18050.068.5775451
AGGGAAT33450.068.141285
AGGGTAG13900.067.958845
AGTAAGG16500.067.888531
TAGACGG4300.067.8621