Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127201.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2619779 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 26587 | 1.014856596682392 | No Hit |
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC | 6751 | 0.25769349246634926 | No Hit |
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG | 4916 | 0.18764941622938422 | TruSeq Adapter, Index 14 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG | 4761 | 0.18173288662898665 | TruSeq Adapter, Index 14 (95% over 21bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 4100 | 0.15650175072019434 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 3292 | 0.12565945448070237 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT | 2833 | 0.10813889263178306 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 8155 | 0.0 | 85.93581 | 1 |
CGTAGGG | 680 | 0.0 | 73.97565 | 2 |
AGGGATG | 3770 | 0.0 | 73.17479 | 5 |
TAGGGCA | 1190 | 0.0 | 73.061646 | 4 |
TAGGGCG | 380 | 0.0 | 72.96812 | 4 |
ATAGGGA | 2335 | 0.0 | 72.25573 | 3 |
AGTAGGG | 4015 | 0.0 | 70.372475 | 2 |
GGTAAGG | 1465 | 0.0 | 70.036156 | 1 |
AGAGGGC | 4135 | 0.0 | 70.01158 | 3 |
ATAGCGG | 540 | 0.0 | 69.72678 | 1 |
TAAGGGA | 2195 | 0.0 | 69.58466 | 3 |
AGGGCAT | 2655 | 0.0 | 69.565315 | 5 |
GTAGGGA | 1915 | 0.0 | 69.45162 | 3 |
TAGCGGG | 1165 | 0.0 | 69.4091 | 2 |
CGAAGGG | 2685 | 0.0 | 68.98678 | 2 |
ATAGAGG | 1805 | 0.0 | 68.577545 | 1 |
AGGGAAT | 3345 | 0.0 | 68.14128 | 5 |
AGGGTAG | 1390 | 0.0 | 67.95884 | 5 |
AGTAAGG | 1650 | 0.0 | 67.88853 | 1 |
TAGACGG | 430 | 0.0 | 67.862 | 1 |