FastQCFastQC Report
Mon 27 Feb 2023
SRR3127200.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127200.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3638664
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395041.085673203131699No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT60430.1660774394118281No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC60320.16577513065234933No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG59270.16288945613005212No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG52270.14365162598140416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110600.084.5085751
ATAGCGG8050.074.75731
GAATAGG23450.073.5805051
TAGGGCA21200.072.493034
ATAGGGC22350.071.2853853
ACGGGAT9700.071.224594
TAGAGGG64950.071.129012
ATAGAGG26100.070.072661
AGGGTAC16200.069.62655
TACGGGT3650.069.531133
ATAGGGA31250.069.481683
GGTAAGG17450.068.704351
GTACGGG8250.068.359632
ACGGGTA4750.068.271554
AATAGGG55700.068.259922
AGTAAGG21450.068.1667561
AGGGAAT34700.068.1266255
AGTAGGG55600.068.044572
CGTAGGG9200.067.9416662
ATAAGGG48500.067.2496642
Produced by FastQC (version 0.11.5)