FastQCFastQC Report
Mon 27 Feb 2023
SRR3127196.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127196.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3321136
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411021.237588584147111No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC69190.2083323296606944No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC68370.2058632949689504No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG60030.18075140554316355No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG59600.1794566678389563No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43470.13088894884160118No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT40670.12245809867467035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129950.085.408321
CGTAGGG9650.076.185182
CTACGAC13850.074.986795
AGGGATG50600.074.0201645
ATAGGGA27550.073.8962253
TACGACC14100.072.990666
TAGGGCA15950.072.9787454
GTAGGGA25200.071.2499543
TAAGGGA29650.070.569723
AGGGCAT27000.070.1428765
ATAGGGC21350.069.971553
AGGGAAT43050.069.317495
AGTAGGG47150.069.266752
GGTAAGG18700.068.579971
GAGGGAT43500.068.5763854
GTAGGGC14500.068.251373
GAATAGG20700.068.103731
CGAAGGG28650.068.100292
TAGAGGG53750.068.038772
TATAGGG24050.067.800712
Produced by FastQC (version 0.11.5)