FastQCFastQC Report
Mon 27 Feb 2023
SRR3127195.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127195.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464669
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432501.2483154956505225No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC72870.21032312177584642No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC68460.19759463313811504No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG58650.16928024004601883No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG57060.16469105706778916No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46680.13473148517217662No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT39740.11470071166971507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT132750.085.2711261
TAGGGCA16350.075.307824
AGGGATG51000.073.073445
ATAGGGC20100.072.4806753
CGTAGGG10000.072.40762
GAATAGG23200.071.6156851
AGGGAAT44700.071.386955
GGTAAGG19150.071.27721
GTAGGGA27100.071.273673
ATAGGGA32550.071.179113
TACGACC15750.070.41876
ATAGCGG6600.069.888151
GAGGGAT43250.069.6511844
CTACGAC16050.069.102465
AGTAGGG53350.068.742232
TAGAGGG54250.068.641842
TAAGGGA29550.068.3860553
AATAGGG49450.068.363782
AGGGCAT29950.068.100375
ATAGAGG22150.066.9357761
Produced by FastQC (version 0.11.5)