FastQCFastQC Report
Mon 27 Feb 2023
SRR3127193.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127193.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550143
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248150.9730826859513368No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54630.21422328081209563No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG39040.15308945419923511No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC38340.15034451009217914No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG37540.14720743111268664No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32150.12607136148835577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79550.083.8733141
TAGGGCA13750.077.121214
AGGGATG53250.075.631315
CGTAGGG6750.072.614532
ATAGGGA26500.072.383993
ATAGGGC19050.071.251083
ACGGGAT8750.071.0984044
GTAGGGC11100.070.906723
GTAGGGA22950.070.848453
AGTAGGG39850.070.6057362
ATAGCGG5950.070.531081
TATAGGG22250.069.476482
TAAGGGA21600.069.167093
GGTAAGG14050.068.463861
AAGGGAT47950.068.409224
AGGGTAC12000.068.140985
CGGGATA5250.068.02915
AGGGAAT33600.067.973155
TAGGGCG5700.067.8005144
TAGAGGG41000.067.5907752
Produced by FastQC (version 0.11.5)