FastQCFastQC Report
Mon 27 Feb 2023
SRR3127192.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127192.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630622
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259580.9867628264342044No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57110.21709694513312822No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG37260.14163950578988543No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC36440.13852237227545425No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG35550.135139141997596No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34110.12966515143566806No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC26680.10142088068905376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82150.083.027181
TAGGGCA13350.076.039284
CGTAGGG6950.075.777142
AGGGATG51100.074.495435
ATAGCGG5500.074.45881
AGGGCAT24300.073.105675
ATAGGGA25500.072.42993
GTAGGGC12350.071.541243
AGGGAAT32800.071.3544545
ACGGGAT8050.071.224514
AGTAGGG40950.071.19392
AGAGGGC39800.071.085163
AGGGCAC21900.069.0998845
GAGGGCA32450.068.937864
GGTAAGG12750.068.300051
ATAGGGC19150.068.2246863
TAGAGGG46000.068.182512
GTAGGGA22100.068.049243
GGATGGC40850.067.3022167
AAGGGAT47850.066.983524
Produced by FastQC (version 0.11.5)