FastQCFastQC Report
Mon 27 Feb 2023
SRR3127191.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127191.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108149
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110760.9995045792578434No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39320.3548259304479813No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG28880.2606147729231358No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG28630.2583587586145907No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC24230.218652906784196Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41500.085.7757641
TCCGCTA6100.084.025961
CGCTACG6150.082.529773
CGAAGGG10500.082.355112
GCTACGA6250.081.9612354
ATAGCGG1900.081.6727451
AGGGATG33000.080.890295
AGCGGGA10450.079.601093
TAGACGG950.079.1978151
ACGGGAT4050.078.907064
AGGGTAC4400.077.970835
AAGGGCA20200.077.47384
TAGCGGG4400.076.902742
GGATGGC19150.076.81357
AATGCGG1900.076.7228851
TAGGGCG2100.076.088954
AAGGGAT35150.076.076224
ACGACCA6800.076.023147
GCGGGAT5150.075.741324
ATAGAGG7400.075.61921
Produced by FastQC (version 0.11.5)