FastQCFastQC Report
Mon 27 Feb 2023
SRR3127189.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127189.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908593
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82590.9089878526468947No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30320.3337027690065849No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG23890.2629340089567056No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG23880.26282394867669023No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC21610.2378402651132025Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGG650.086.8640441
TAGGGCG1800.086.156384
CGTTTTT31550.085.7512741
TCCGCTA5600.083.180081
CGCTACG5650.083.175913
AGGGTAC3300.082.59625
TACGCGG401.02591E-982.339861
AGGGATG27250.082.089285
AGCGGGA7300.081.7573553
AAGGGCA16550.080.926894
CTACGAC5850.080.332285
GCTACGA5850.080.332284
TAGACGG1300.079.6253741
CGAAGGG7450.079.506692
CGGGATA1850.078.7473455
GACCGTA301.4569287E-678.323988
ACGACCA5700.078.323987
TACGACC5950.078.192346
AAGGGAT29750.077.402524
AGGGCAT11050.077.402515
Produced by FastQC (version 0.11.5)