FastQCFastQC Report
Mon 27 Feb 2023
SRR3127188.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127188.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191827
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179521.506258878176111No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC66530.5582186005183638No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG60650.5088825811128629RNA PCR Primer, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG55350.4644130398119862RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC49170.41255987655926574RNA PCR Primer, Index 38 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT21190.17779426040859955No Hit
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT18320.1537135842702003RNA PCR Primer, Index 38 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT18070.15161596439751743RNA PCR Primer, Index 38 (95% over 21bp)
TAGACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT16160.13559014857022034No Hit
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG14550.12208147659014269RNA PCR Primer, Index 27 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT14490.12157804782069882RNA PCR Primer, Index 38 (95% over 21bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC13550.11369099709941123No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCGA12470.10462927924942127No Hit
ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT12210.10244775458183109RNA PCR Primer, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55900.087.1191
CGTAGGG2100.082.811892
CGGGTAT1600.082.252355
AGGGATG31450.081.897415
CGAAGGG7350.081.8526842
CTAGCGG1150.081.7517551
TCCGCTA13000.081.7203141
CGCTACG12600.081.696243
GCTACGA12750.080.73514
GGATGGC22750.079.953937
AACGGGC2850.079.1634753
CTACGAC13100.078.574765
ACGGGTC1500.078.338864
TAGGGCA6700.077.871164
AGGGAAT25900.077.851655
TAAGCGG2450.076.7465441
TACGACC13500.076.594786
CGACCAA13300.076.333018
AGGGCAT11700.076.326975
GAATGCC13550.075.965278
Produced by FastQC (version 0.11.5)