FastQCFastQC Report
Mon 27 Feb 2023
SRR3127187.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127187.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992007
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127631.2865836632201184No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG57460.5792297836608008RNA PCR Primer, Index 27 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49680.5008029177213468No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG49600.4999964717990901RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC42810.43154937414756145RNA PCR Primer, Index 38 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT18660.18810351136635126No Hit
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT18490.18638981378155597RNA PCR Primer, Index 38 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT17690.17832535455899RNA PCR Primer, Index 38 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT15760.1588698466845496RNA PCR Primer, Index 38 (95% over 21bp)
TAGACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT13890.14001917325180166No Hit
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG12690.12792248441795268RNA PCR Primer, Index 27 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCGA12350.12449508924836217No Hit
GGAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT11420.1151201554021292No Hit
AGAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT10620.10705569617956326No Hit
ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT10370.10453555267251138RNA PCR Primer, Index 27 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG10260.10342668952940856RNA PCR Primer, Index 27 (95% over 22bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10150.10231782638630575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGA156.8245776E-494.266922
CGTTTTT41500.087.068571
CTAGCGG1150.086.139471
CGCTACG9450.084.790353
GGATGGC17800.083.162297
GCTACGA9750.082.181414
TCCGCTA9950.082.0169751
AGGGATG27750.081.2857065
CTACGAC9850.081.105195
TACGACC9900.080.220886
CGGGATA1650.079.74625
ACGGGAT2700.078.555764
TAGAGGG10750.078.482692
CGAAGGG7300.078.125332
GCGGGAT4000.077.770214
AACGGGA3700.077.706513
ACGACCA10050.077.153177
TAGGGCA4950.077.127494
CGTAGGG2200.077.127482
GATGACC14150.077.049088
Produced by FastQC (version 0.11.5)