Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127185.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 747351 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 10150 | 1.3581302493741227 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 4076 | 0.545392994724032 | No Hit |
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG | 2535 | 0.33919804750378335 | No Hit |
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG | 1980 | 0.26493575307987816 | No Hit |
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC | 1956 | 0.2617244106183038 | No Hit |
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC | 1267 | 0.16953212078394223 | No Hit |
AAAGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTT | 1102 | 0.14745414136061902 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT | 814 | 0.10891803182172767 | No Hit |
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT | 753 | 0.10075586973189304 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTAGGG | 205 | 0.0 | 89.4014 | 2 |
CGTTTTT | 3160 | 0.0 | 87.35833 | 1 |
TAGGGCG | 210 | 0.0 | 82.80835 | 4 |
GCTACGA | 720 | 0.0 | 82.24883 | 4 |
AGGGCAT | 700 | 0.0 | 81.2417 | 5 |
GGATTCG | 35 | 3.8631697E-8 | 80.570274 | 7 |
CGCTACG | 740 | 0.0 | 80.02054 | 3 |
ACGGGTG | 125 | 0.0 | 78.95888 | 4 |
TAGACGG | 60 | 0.0 | 78.37942 | 1 |
GACCGAT | 30 | 1.4557645E-6 | 78.332214 | 8 |
AGGGAAT | 1715 | 0.0 | 78.10384 | 5 |
GTAGGGA | 730 | 0.0 | 77.89779 | 3 |
AGGGATG | 1820 | 0.0 | 77.47143 | 5 |
TCCGCTA | 760 | 0.0 | 77.348114 | 1 |
TGTAGGG | 565 | 0.0 | 76.519646 | 2 |
ACGACCA | 775 | 0.0 | 76.41182 | 7 |
GGATGGC | 1435 | 0.0 | 76.3125 | 7 |
GTAGGGC | 345 | 0.0 | 76.28366 | 3 |
ACGGGAT | 255 | 0.0 | 75.56755 | 4 |
GGATGAC | 840 | 0.0 | 75.53464 | 7 |