FastQCFastQC Report
Mon 27 Feb 2023
SRR3127185.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127185.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747351
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101501.3581302493741227No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40760.545392994724032No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG25350.33919804750378335No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG19800.26493575307987816No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC19560.2617244106183038No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC12670.16953212078394223No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTT11020.14745414136061902No Hit
CGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT8140.10891803182172767No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT7530.10075586973189304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2050.089.40142
CGTTTTT31600.087.358331
TAGGGCG2100.082.808354
GCTACGA7200.082.248834
AGGGCAT7000.081.24175
GGATTCG353.8631697E-880.5702747
CGCTACG7400.080.020543
ACGGGTG1250.078.958884
TAGACGG600.078.379421
GACCGAT301.4557645E-678.3322148
AGGGAAT17150.078.103845
GTAGGGA7300.077.897793
AGGGATG18200.077.471435
TCCGCTA7600.077.3481141
TGTAGGG5650.076.5196462
ACGACCA7750.076.411827
GGATGGC14350.076.31257
GTAGGGC3450.076.283663
ACGGGAT2550.075.567554
GGATGAC8400.075.534647