FastQCFastQC Report
Mon 27 Feb 2023
SRR3127184.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127184.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615931
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76791.2467305591048348No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28700.4659612846244141No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG25540.4146568365612382No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG21430.3479285829094493No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC20880.33899901125288384No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTT11330.18394917612524778No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC9860.16008286642497294No Hit
CGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT8190.13296943975867426No Hit
AGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT7400.12014332774288028No Hit
GGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT6740.10942784175500178No Hit
AAAGCGGCTGTGCAGACACTGTCTCTTATACACATCTGACGCGGCAGACT6460.10488187800256846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT24150.086.68631
CGTAGGG1150.086.086562
ATAGCGG1100.085.727861
AGGGCAT6450.085.243815
GACGGGT452.7284841E-1183.802323
TAGCGGG2550.083.19292
AACGGGC1600.079.546723
TCCGCTA5600.079.145181
TCGAGGG2750.078.856782
GGATGAC6450.078.686597
GGTTAGC301.456554E-678.322317
GCTACGA5600.078.284094
AGGGATG15300.077.400865
CGCTACG5700.076.9106753
GGATGGC10750.076.5008547
GATGACC6400.076.364248
AAGGGCA8300.076.1036154
TAACGGG1550.076.036522
ATAGGGA7350.075.678623
TAAGGGA6300.075.571733