FastQCFastQC Report
Mon 27 Feb 2023
SRR3127183.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127183.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624416
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78281.2536514118792599No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30510.4886165633167632No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG24440.39140572951365754No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG20100.32190078409265616No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC19780.31677599549018604No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTT10580.1694383231691693No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10090.16159099062163687No Hit
CGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT8090.12956106185619845No Hit
AGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT7120.1140265464049608No Hit
GGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT6440.10313637062471175No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC156.9044635E-493.987184
TAGTGCG156.9044635E-493.987189
CGTTTTT25600.086.7416841
AGGGCAT6050.080.782385
TAGCGGG2400.080.3321842
CGTAGGG1350.080.114482
CGAAGGG5200.079.57862
GCGGGTA1250.078.9492344
ACGGGAT2750.078.607474
TCCGCTA5450.078.5544361
CTAGCGG600.078.410561
AGGGATG15900.078.3226555
GCTACGA5350.078.176264
GTAGGGC2950.078.057163
GGATGGC12650.077.2700967
GACGGGT551.8189894E-1276.8986053
GGAATGC7000.076.5324257
AGGGAAT11900.076.216515
CGCTACG5650.075.68883
AAGGGTA4850.075.5773244