FastQCFastQC Report
Mon 27 Feb 2023
SRR3127182.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127182.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575749
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402131.1246035446000264No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT94990.2656506371112738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT125650.084.1673051
ATAGGGC21850.073.349063
TAGACGG5100.070.9965061
AGTAGGG56400.068.33142
ATAGGGA33000.067.792983
AGGGACT57650.067.502945
TAGTAGG15750.067.475221
GTAGGGA28550.066.8361743
AATAGGG51550.066.828372
GTATAGG12950.066.8135451
TAAGGGA31950.066.784653
AGAGGGC50950.066.693843
GAATAGG21150.066.477891
TAGAGGG64350.066.31672
GATAGGG51350.066.081872
CGTAGGG11200.065.882072
AGGGATG48550.065.634495
AGTAAGG22000.065.1918951
GAGGGAT43800.065.136514
ACGGGAT8950.065.119124
Produced by FastQC (version 0.11.5)