FastQCFastQC Report
Mon 27 Feb 2023
SRR3127181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765055
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301201.089309254246299No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT73500.2658174973011387No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG30040.1086416002574994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90800.086.211421
TAGACGG4400.075.021871
ATAGGGA26100.071.507723
ACGGGAT6000.071.4806444
ATAGGGC16850.071.324473
CGTAGGG9050.069.785052
AGAGGGC40350.069.614713
AGTAAGG15750.068.564211
GAATAGG14450.068.532091
TATAGGG21050.068.5134052
AGTAGGG43950.068.4176252
GGTAAGG17400.068.024761
CGAAGGG27200.067.230912
AGGGATG37600.067.116065
ATAAGGG37100.066.821782
GATAGGG41500.066.778312
AGGGACT46900.066.031715
TAGAGGG48350.065.993082
AATAGGG36300.065.957362
AAGAGGG101050.065.810762
Produced by FastQC (version 0.11.5)