FastQCFastQC Report
Mon 27 Feb 2023
SRR3127180.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127180.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895808
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313271.0818051473025836No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT78530.27118510619488584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93200.085.323991
TAGACGG3900.076.010981
TAGGGTA8600.072.1345144
TAGTAGG12600.071.3285141
ATAGGGC17700.070.893373
GAATAGG15700.069.2329251
ATAGGGA25000.068.803213
ATAGGGT10400.068.687533
AGTAGGG45350.068.631082
CGTAGGG8400.067.7245562
TAGCGGG15150.067.3421862
TATAGGG21600.067.258142
AGAGGGC43200.067.0138553
GGTAAGG17100.066.86681
TAAGGGA24750.066.64993
AAGGGGC89200.066.3328254
GAGGGAT35000.066.198254
ATAGCGG6900.066.148961
AATAGGG37200.066.099612
ACGGGAT6900.066.067864
Produced by FastQC (version 0.11.5)