FastQCFastQC Report
Mon 27 Feb 2023
SRR3127179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367327
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160651.1749201178650022No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG35320.2583142145222028No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG32020.23417953422992452No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC29840.21823601815805582No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC15800.11555392382363545No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.10004922012071729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51100.085.934421
GGTAAGG7850.079.656851
AGGGATG31650.079.147255
ATAGCGG2200.079.071541
AGGGAAT24850.078.1098865
AGGGTAC5750.077.64945
TAAGGGA15100.075.007823
GGAATGC15450.073.3112957
TAAACGG2200.072.660341
ACGGGTA1300.072.305114
GAGGGAT22100.072.305114
GTAAGGG20450.072.161042
TAGGGCA9900.072.1590354
ACTAGGG8200.071.641162
ATGAGGG20900.071.281922
GGGAATG28550.071.279416
GGATGGC25000.071.249457
CGAGGGA8650.070.087413
TGTAGGG9100.069.720232
TAGGGCG2300.069.475784
Produced by FastQC (version 0.11.5)