FastQCFastQC Report
Mon 27 Feb 2023
SRR3127178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111782
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116261.0457086011466277No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG35540.31966698507441205No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG30880.27775229316538674No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC27790.2499590747106897No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC12930.1162997781939265No Hit
AGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT12140.10919406862136642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT36500.087.489921
GGTAAGG5250.080.8621751
AGGGATG25100.080.5068365
CGAGGGA7200.076.591833
CGTAGGG2850.076.075012
AGGGAAT18700.075.6419755
AGGGCAC8300.074.736695
TAGGGCA7650.074.550914
GGATGGC20550.072.9485867
TAGCGGG5850.072.512972
TAAGGGA11600.072.325443
AACGAGG3150.071.877491
TACGGGT1050.071.8223653
GTAGGGC5600.071.541813
AGTAAGG6500.071.117251
AAGGGTA6850.070.872164
GGCGATG3250.070.8517767
AGTAGGG17050.070.769262
GTAGCGG3150.070.3800351
TATAGGG9050.070.309532
Produced by FastQC (version 0.11.5)