FastQCFastQC Report
Mon 27 Feb 2023
SRR3127176.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127176.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838542
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196331.0678570301902268No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC77150.41962598624344727No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG61100.33232855164581504No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG57640.3135092916017148No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC56830.30910362667809604No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT25640.1394583316562798No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC22000.11966003496248659No Hit
AGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT21820.1186809983127935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG2300.087.863754
CGTTTTT63400.085.28151
ATAGCGG4400.084.4152151
AGGGATG50650.083.879935
CTAACGG1300.083.182271
TCCGCTA15700.078.75941
ACGGGAT6450.078.692474
CTACGAC15850.078.575235
TAGCGGG9700.078.489652
CGAGGGA12950.078.025723
CGAAGGG14400.078.001782
TACGACC16000.077.544866
GGATGGC36900.077.309247
CGCTACG16050.077.010473
TAACGGG4100.076.799792
GCTACGA16300.076.117654
TAAGGGA25550.075.599693
AAGGGAT50750.075.472834
CGTAGGG4900.074.811372
TACGAGG3400.074.672581