FastQCFastQC Report
Mon 27 Feb 2023
SRR3127174.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127174.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1554925
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149280.9600463044841391No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61460.39526022155409424No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG55650.35789507532517645No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG49060.3155136099811888No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC47430.30503078926636334No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT22940.1475312314098751No Hit
AGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT18550.1192983584417255No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC18540.11923404665819895No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT17510.11260993295496566No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50300.085.3090741
AGGGATG42250.083.4257055
CTAGCGG1200.082.3391651
ACGGGAT6300.079.819414
GCGTAGG1900.079.24371
CTACGAC12550.079.0145
GGATGGC30350.078.508127
CGAAGGG11000.078.2127152
CGCTACG12650.078.017873
TACGACC12750.077.774566
TCCGCTA12950.077.7521441
AAGGGAT40550.076.840114
CGAGGGA11650.076.6466143
GCTACGA12950.076.573414
ACGGGTA2100.076.089544
GAGGGAT30950.075.771384
GCGGGAT8950.075.614454
AAGGGCG5300.075.371714
CGTAGGG3950.074.982932
ACGACCA12850.074.974937