FastQCFastQC Report
Mon 27 Feb 2023
SRR3127172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080888
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127041.1753299139226265No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC64960.6009873363382701No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG39360.36414503630348377No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG34150.31594392758546674No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC32730.3028065812554122No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGAC15100.13969995041114344No Hit
AAAGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT14480.13396392595717596No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT13010.12036399700986597No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC12340.11416538993864304No Hit
AGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT11540.10676406806255598No Hit
GGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT11130.10297089060106136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGG900.089.0344851
CGTTTTT40550.086.832351
GCTACGA11950.083.99684
CTACGAC12000.083.414655
CGCTACG12100.082.955533
TACGGGA2500.082.939953
TCCGCTA12200.082.681011
ACGACCA11900.081.746167
ATAGCGG1500.081.702231
ACGGGAT5000.081.0549554
AGGGATG29950.080.808015
CGTAGGG2450.080.785672
AGGGAAT23200.079.809075
TAGGGCA5050.079.319264
TAAGGGA12850.078.48053
TAGGGCG2300.077.858654
GTACGGG2000.077.75622
CGAAGGG7700.077.72562
AAGGGTA7450.077.171094
GGAATGC16450.077.1332867