FastQCFastQC Report
Mon 27 Feb 2023
SRR3127171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112777
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131161.1786728158472002No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC70040.6294163161172454No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG36120.32459333720952177No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG34280.3080581284480179No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC32000.2875688480261544No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGAC16160.14522226825320797No Hit
AAAGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT14120.1268897541915406No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC13680.122935682531181No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT12100.10873697065988963No Hit
AGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT11180.1004693662791377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT40700.084.898281
CTACGAC13850.084.833015
TAGGGCG1350.083.551094
CGCTACG14200.082.411073
ACGGGAT4600.081.734754
GCTACGA14450.081.3105244
AGGGAAT23400.081.140955
AGGGATG31050.079.918435
ACGACCA14300.079.862867
TCCGCTA14800.079.837921
GGAATGC18000.079.634637
GGATGGC23300.077.6567847
AGGGCGT1700.077.407625
TAGGGCA6450.077.2361764
AGGGCGC2800.077.210155
ATAGCGG1650.077.033041
TACGACC15300.076.7932746
AGGGTAT6750.076.5884865
CCGCTAC15500.076.463782
ATAGGGA14700.076.410873
Produced by FastQC (version 0.11.5)