FastQCFastQC Report
Mon 27 Feb 2023
SRR3127170.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127170.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800935
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268350.958072929218279No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59890.21382145604949776No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG48710.17390621346086219No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG43050.15369867562081949No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC42010.14998562979862082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81600.082.793911
ATAGGGA22250.076.677643
AGGGCAT26150.072.61095
AGTAGGG41850.071.537892
ATAGCGG6600.071.234081
ATAGGGC18050.071.0849463
TAGGGTA9950.070.382244
TAAGGGA27850.070.3725053
ACGGGTC2550.070.039554
TATAGGG21800.069.8522952
AGGGAAT35600.069.8389745
AGGGATG41650.069.6245355
TAGCGGG16700.069.232632
TAGAGGG52650.068.7360462
AGGGACT48400.068.362745
TAGACGG3650.068.2676241
ATAGAGG20950.068.2215041
TAGGGCA15300.068.19644
ACGGGAT7250.068.069294
GTAGGGA21750.068.068073
Produced by FastQC (version 0.11.5)