FastQCFastQC Report
Mon 27 Feb 2023
SRR3127169.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127169.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249140
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198510.8826040175355914No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47800.2125256764807882No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG46940.2087019927616778No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG38880.17286607325466624No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC37270.1657077816409828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64200.085.177221
TAGACGG2950.075.106291
TAGCGGG12650.074.493312
TAAGGGA19900.073.871913
ACGGGAT6400.072.884144
AGGGAAT29050.072.635165
ATAGGGC16300.072.554433
TAGGGCA11800.072.272714
AGGGCAT18850.071.800565
AGGGATG33750.071.292345
ATAGCGG5250.070.936261
AGATAGG12300.070.9034041
AGTAGGG34450.070.7097242
ATAGGGA18350.070.6113363
AAGGGCG9000.070.1524
TAGAGGG38750.069.55082
GTAGGGC10400.069.3163763
ACGGGTC3500.068.656234
GTACGGG4750.068.443672
AGGGACT37150.068.436085
Produced by FastQC (version 0.11.5)