FastQCFastQC Report
Mon 27 Feb 2023
SRR3127168.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127168.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2355728
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210230.8924205171395No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51260.2175972777841924No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG47410.20125413460297623No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG37820.16054485067885596No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC37110.15753092037790442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66000.084.124091
ACGGGAT7600.078.535344
ATAGGGA19900.074.3930743
AGGGAAT29150.073.680525
GTACGGG3650.073.4230042
TAGCGGG12100.072.273282
ATAGGGC15500.071.860733
TAGGGCA12850.071.6849444
AGTAGGG33550.070.910112
GTAGGGT9000.069.97413
TAGGGAC13400.069.794834
TACGGGT1550.069.7382663
ATAGCGG5050.068.948161
AGGGATG35400.068.903155
TAAGGGA23000.068.8615953
TAGACGG3350.068.8231
GAATAGG14450.068.055111
TATAGGG16900.067.88182
GGTAAGG13800.067.510051
GATAGGG33400.067.427832
Produced by FastQC (version 0.11.5)