FastQCFastQC Report
Mon 27 Feb 2023
SRR3127167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131438
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT604411.9301356118179573No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47080.15034626264355225No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT42070.1343472232246016No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT32590.10407359174922193No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG31720.10129531544293707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT182950.087.989581
GAATAGG17000.070.818671
ATAGGGA27250.070.538973
ATAGGGC16000.070.2021263
AGGGCAT27200.067.905185
AGAGGGC48150.067.5432743
AGTAGGG47300.067.166222
TAGGGAT19600.066.6625444
ATAGCGG7500.066.466281
TAGAGGG56100.066.4317552
TAGCGGG16750.065.935372
GTAGGGC12850.065.83273
GTAGGGA24000.065.7961353
AAGAGGG110650.065.705892
AGGGATG43050.065.284075
TAGGGCA14150.065.101764
AGTAAGG21400.065.048111
TAAGAGG24450.065.012161
CGTAGGG9100.064.555622
ATGAGGG51050.064.44182
Produced by FastQC (version 0.11.5)