FastQCFastQC Report
Mon 27 Feb 2023
SRR3127165.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127165.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2565624
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474341.8488289788371173No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39630.15446534644203516No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT38000.1481121161947347No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT31010.12086728218944007No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG28060.1093691047480067No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT27760.10819979856752197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT144700.087.179411
GTAGGGC10850.075.369913
ATAGGGC14250.070.909123
GAATAGG15000.069.977971
TAGGGCA11700.069.0909354
ATAGGGA22400.068.608543
ATAGAGG18800.068.352191
AGTAGGG38150.068.276542
AAGAGGG86450.066.9498142
TATAGGG18450.066.766892
AGGGATG33150.066.491765
AGATAGG12900.066.409371
CGTAGGG6800.066.377152
AAGGGGC82800.065.785724
ATGAGGG40350.065.719142
TAGAGGG46450.065.3887562
AGAGGGC37500.064.919783
TAGGGAT14050.064.5593954
AGGGAAT25800.064.48555
ACGGGTA2700.064.404544
Produced by FastQC (version 0.11.5)