FastQCFastQC Report
Mon 27 Feb 2023
SRR3127164.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127164.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947895
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185970.9547229188431615No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC77670.3987381250016043No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG58510.3003755335888228No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG52480.2694190395272846No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC52340.2687003149553749No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT28150.14451497642326716No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC22140.11366115730057319No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66050.085.1384051
AGGGATG42350.079.2400365
ACGGGAT7450.078.228554
TAGCGGG7100.078.105022
AGGGAAT33250.077.744725
ACGGGTA2600.077.731134
CGAAGGG16200.077.455372
CGCTACG14050.076.270823
AAGGGCA35500.075.5975344
TAGGGCA14450.075.4605564
TAAGCGG4300.075.448011
AAGGGAT44950.075.0748754
AGGGCAT25400.074.571215
GTAGGGC10700.074.2339863
GGATGGC28000.074.025347
GAAGGGC28400.073.81023
AGAGGGT18550.073.73083
TCCGCTA14800.073.70441
CGAGGGA13800.073.565563
GCGGGAT10400.073.2118764
Produced by FastQC (version 0.11.5)