FastQCFastQC Report
Mon 27 Feb 2023
SRR3127163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591345
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131720.827727488382469No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC59870.3762226292852901No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG54750.3440485878297918No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG44150.27743826762895535No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC42850.26926907741564526No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT25030.15728833156857877No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT18530.11644238050202815No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC17430.10952998878307343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48650.084.479221
ACGGGTA2350.082.196694
AGGGATG34300.079.191585
AGGGAAT28050.078.742695
AGTAAGG9550.078.4711461
GGTACGA600.078.323857
TAGGGCA11450.078.178344
CGAAGGG10850.077.727612
GTACGGG3100.077.51052
CGTAGGG3350.077.351712
CGAGGGA11400.077.281423
AAGGGCG8100.076.776314
GAAGGGC22500.076.427323
TAACGGG6500.076.10762
GGTAAGG9250.074.901731
AAGGGCA29100.074.473754
AGGGTAC7650.074.330875
CGCTACG11800.074.262463
TAGCGGG5650.074.2153242
AAGGGAT33650.074.064314