FastQCFastQC Report
Mon 27 Feb 2023
SRR3127161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579769
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149580.9468472922306995No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC91740.5807178138069553No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG38870.24604863116063172Illumina PCR Primer Index 8 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG32460.20547307865896852Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC28790.18224183409093353Illumina PCR Primer Index 8 (95% over 23bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC20400.12913280359343676No Hit
AAAGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT19870.12577788271576415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50100.086.239241
CGCTACG15900.083.652513
TCCGCTA16200.083.290231
ATAGCGG2550.082.9658661
ACGGGAT8550.082.454584
AGGGATG54900.081.0713355
CGGGTAT2800.080.569915
CGGGAAT7350.080.569915
GCTACGA16500.080.325764
CGTAGGG5700.079.978422
CTACGAC16850.079.2151265
ACGACCA16550.078.381677
AAGGGAT53850.078.2882164
AACGGGA9850.077.7751853
CGACCAA16700.077.6776358
GGATGGC35300.077.624227
TACGACC17150.077.555396
GAGGGAT33600.077.212834
GTACGGG3550.076.784862
TAAGGGA25600.076.1899643