SRR3127160.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3127160.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1252939
Sequences flagged as poor quality	0
Sequence length	100
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11065	0.8831235997921686	No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	6876	0.5487896856910033	No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	3841	0.30655921796671665	Illumina PCR Primer Index 8 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	2908	0.23209429988211716	Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	2714	0.21661070491061415	Illumina PCR Primer Index 8 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT	1874	0.14956833493091046	No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC	1557	0.12426782149809368	No Hit
AGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT	1485	0.1185213326426905	Illumina PCR Primer Index 8 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCAGG	1368	0.10918328825266035	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	120	86.44165	1
CGTTTTT	3810	85.26599	1
AGGGATG	4270	83.09211	5
CGGGATG	935	81.92504	5
CGCTACG	1185	81.52832	3
TCCGCTA	1210	80.66155	1
AAGGGAT	3905	80.496635	4
GCGGGAT	975	79.753975	4
GGATGGC	2925	79.68865	7
ACGGGAT	670	79.48344	4
ATAGCGG	315	79.331726	1
TAAGGGA	1775	78.85534	3
TAGGGCA	720	78.54558	4
CGTAGGG	410	78.16243	2
CGACCAA	1205	77.99803	8
GCTACGA	1230	77.77928	4
AGGGTAC	345	77.64196	5
TACGACC	1235	77.62541	6
CTACGAC	1250	77.44581	5
GAGGGAT	2695	77.11748	4